Effect of drought stress and bio-fertilizers and chemical fertilizers on some morphplogical and physiological characteristis of linseed (Linum usitatissimum L.)

Document Type : Research Paper

Authors

1 student/shahrekord university

2 Associate Professor/Shahrekord University

3 Msc./tehran university

Abstract

In order to evaluate the effects of drought stress, bio-fertilizers and chemical fertilizer (N+P) treatments on some traits of linseed, include percent of proline, potassium and calcium and the pattern of CGR, a field experiment was conducted as split-plot in RCBD design with three replications at Shahrekord University in 2013.Drought stress at four levels of without stress (with potential of 0.3 atmosphere), light stress(with potential of 3.5 atmosphere), moderate stress(6.5 atmosphere) and severe stress (9.5 atmosphere) as main factors and five fertilizer treatments of control (no ferlilizer), chemical fertilizer (N+P), bio-fertilizer of Azotobarvat 1, Azotobarvar2 and combination of Azotobarvar 1+ Azotobarvar 2 as minor factors were used. Results showed that the effects of drought stress, fertilizer and their interaction on the characteristics of proline, calcium and potassium concentrations were significantat 1percent level (P≤0.01).The maximum proline (19.24 mg/g dry weight) and potassium (3.10%) obtained under severe drought stress and Azotobarvar 1+ Azotobarvar2 treatments and maximum calcium obtained under severe drought stress and Azotobarvar2 treatments. Analyzing of the trend of crop growth rate the maximum CGR observed at65days after seedling emergence in soil in both control treatment of without stress and chemical fertilizers(N + P).Because ofthe side environmental effects of using chemical fertilizers, using bio-fertilizers of azotobarvar 1, Azotobarvar 2 is recommended.

Keywords


[1].   Abdul-Majid, S., Rehana, A., & Ghulam, M. (2007). Potassium-calcium inter relationship linked to drought tolerance inwheat, Pakistan Journal of Botany, 39(5),1609-1621.
[2].        Afrasiabi, M.,AminiDehaghi, M., & ModaresSanavi, A. (2011). Effect of phosphate biofertilizer Barvar-2 and triple super phosphate fertilizer on yield, quality and nutrient uptake of Medicagoscutellata, cv. Robinson, Journal of Agronomy Sciences, 2(4), 43-54, ( in Farsi).
[3].        Alikhani, H.A., & Yakhchali, B. (2009). Potential use of Iranian rhizobial strains as plant growth-promotingrhizobacteria (PGPR) and effects of selected strains on growth characteristics of wheat, corn and alfalfa. Desert, http://jdesert.ut.ac.ir.
[4].        Alizade, A. (2008). Plantsand soil water relations, Press of Astan Qods Razavi, Mashhad, 396 pp.
[5].        Alizade, O., & Alizade, A. (2007). Mycorrhizal effects on nutrient uptake of maize under different soil moisture, Journal of Agricultural Sciences, 3:1, 101-109, (in Farsi).
[6].        Bates, L.S., Waldren, R.P., & Teare, L.D. (1973). Rapid determination of free proline for water-stress studies, Plant and Soil, 39, 205-207.
[7].        Darzi, M.T., Ghalavand, A., & Rejali,  F. (2009). The effects of biofertilizers application on N, P, K assimilation and seed yield in fennel (Foeniculum vulgare Mill.), Iranian Journal of Medicinal and Aromatic Plants, 25(1), 1-19, (in Farsi)
[8].        Eidizade, K.H., MahdaviDamghani, A., Sabahi, H., & Sufi zade, S. (2010).  Effect of Biological fertilizer in combination with inorganic fertilizer on the growth of maize (Zea mays L.) in Shoushtar, Journal of Agricultural Ecology, 2(2), 292-301, (in Farsi).
[9].        Emami, A. (1996). Analysis methods of plant. Soil and water research institute press. 202pp.
[10].    Fanaei, H.R., Galuy, M., Kafi, M., & Shirani rad, A.H. (2013). Interaction of Water Deficit Stress and Potassium Application on Potassium, Caldcium, Magnesium Concentration and Oil of Two Species of Canola (Brassica napus) and Mustard (Brassica juncea),  Journal of Soil and Water, 23(3), 261-275, (in Farsi).
[11].    Ghorbanli, M., BakhshiKhaniki, G.H., & Zakeri, A. (2011). Effect of drought stress on anti oxidan compound on linseed (Linum usitatissimumL.), Iranian Journal of Medicinal and Aromatic Plants, 27(4), 646-658, (in Farsi).
[12].    Girousse, C., Bournolille, R., & Bonnemain, J.L. (1996). Water deficit changes in concentraition of proline and som other amino acids in the phloem sap of alfalfa, Plant Physiology, 111, 109-115.
[13].    Hamidi, H., & Safarnejad, A. (2002). The study Morphological and biochemical characteristics of the callus of alfalfa under drought stress, Research and Development Journal, 58, 84-89, (in Farsi).
[14].    Hernandez, A.N., Hernandez, A., & Heydrich, M. (1995). Selection of rhizobacteria for use maize cultivation, Journal of CultivosTropicales, 6, 5-8.
[15].    Jafarzadeh, L., Omidi, H., & Bostani, A. A. (2013). Effect of drought stress and bio-fertilizer on flower yield, hotosynthesis pigments and proline content of Marigold (Calendula officinalis L.), Iranian Journal of Medicinal and Aromatic Plants, 29(3), 666-681, (in Farsi).
[16].    Kafi, M. (1995). Plant Physiology (Volume II), Mashhad Press University Jihad, 676 pp, (in Farsi).
[17].    Khajeh­Pour, M.R. (2013). Principles of agronomy. Esfahan University of Jihad press, 658 pp, (in Farsi).
[18].    Khan, M.M.A. (2006). In Glimpses in plan Research, Medicinal Plants, 6. 287-290.
[19].    KhanpourArdestani, N., ZareMarivan, H., & Ghanati, F. (2008). Potassium and magnesium uptake in mycorrhizal maize plants under different amounts of potassium and magnesium, Plant and Ecosystem journal, (14), 87-101,(in Farsi).
[20].    Khoramdel, S., Kuchaki, A., NasiriMahalati, M., & Ghorbani, R. (2010). Effect of fertilizers on the yield and yield components of biological medicinal NS, Iranian Journal of Field Crops Research, 8(5), 758- 766, (in Farsi).
[21].    Kuchaki, A., & Sarmadnia, G.H.M. (2012). Physiology of Crop Plants, Mashhad Press University Jihad, 400, (in Farsi).
[22].    Kuzentsov, V.I., &S hevyakova, N.I., (1999). Proline under stress: biological role, metabolism, and regulation, Russian Journal of Plant Physiology, 46, 274-287.
[23].    Ma, Q.F., Turner, D.W., Levy, D., & Cowling, W.A. (2004). Solute accumulation and osmotic adjustment in leaves of Brassica oilseeds in response to soil water deficit, Australian Journal of Agricultural Research, 55, 939-945.
[24].    Matsumoto, T.K., Ellsmore, A.J., Cessna, S.G., Low, P.S., Pardo, J.M., Bressan, R.A., & Hasegawa, P.M. (2002). An osmoticallyinduced cytosolic Ca2+ transient activates calcineurin signaling to mediate ion homeostasis and salt tolerance of Saccharomyces cerevisiae. Journal of Biological Chemistry 27. 3075-3080.
[25].    Mirshekari, M., Amiri, R., Irannezhad, H., Noori, A., & Zandevakili, O.R. (2012). Effect of Planting Date and Water Dificit on Quantitative and Qualititative Traits of Flax seed. American-Eurasian Journal of Agriculture and Environmental Science 12(7). 901-913.
[26].    Mirzakhani, M., Ardakani, M.R., Ayeineband, A.,Shirani rad, A.H., & Rejali, F. (2008). Effect of Azotobacter inoculation and mycorrhiza in nitrogen and phosphorus on yield and yield components of spring safflower. The10th Iranian Crop Sciences Congress, 30-28 August, Seed and Plant Improvement Institute, Karaj. PP413, (in Farsi).
[27].    Nazeri, P., Kashani, A., Kavazi, K., Ardakani, M., & Mirakhori, M. (2012). Effect of Use Microbial Zinc Granulated Phosphorous Bio fertilizer on Growth Indices of Bean. Journal of Agronomy and Plant Breeding, 8(3). 111-126. (in Farsi).
[28].    Omidi, A. (2009). Effect of Drought Stress at Different Growth Stages on Seed Yield and some some agronomic and physiological traits of three cultivars of spring safflower. Seed and Plant Production Journal, 25(1), 15-31(in Farsi).
[29].    Rahimi, M., Mohamadi, G.H., Aeinehband, A., Afshar, A., & Maafpourian, G.H. (2009). Effects of Sowing Time and Different Nitrogen Levels on Quantitative and Qualitative Charcteristics of Oil Flax (Linum usitatissimum L.), Seed and Plant Production Journal, 25-2 (1), 79-91, (in Farsi).
[30].    Safarnejad, A. (2003). A review of various methods of selective plants for drought tolerance, Journal of Agricultural Drought and droughts, 7, 7-13, (in Farsi).
[31].    Sajedi, N.A., Ardakani, M.R., Sajedi, A., & Bahrami, A. (2010). Absorption of some nutrients is influenced by mycorrhiza and different levels of drought stress and zink on corn.  Journal of Agricultural Research, 8(5), 784-791, (in Farsi).
[32].    Singh, S., & Kapoor, K. (1988). Effect ofinoculation of phosphate solubilizing microorganisms and arbuscular mycorrizal fungus on mung bean grown under natural soil condition, Mycoorrhiza, 7(5), 249-253.
[33].    Tarigholeslami, M., Zarghami R., Akbar bujar, M., & Oveisi, M. (2012). Effect of Nitrogen fertilizer and Water Deficit Stress on Physiological indices of corn (Zea mays L.), Journal of Agriculture and Plant Modification, 8(1), 161-174, (in Farsi).
[34].    Valadabadi, A., & Ali-abadiFarahani, H. (2008). Effect of potassium application on quantitative characteristics and root penetration of corn, sorghum and millet under drought stress, Journal of Agronomy and Plant Breeding, 4(2), 37-42, (in Farsi).
[35].    Vessy, K. (2003). Plant growth promoting rhizobacteria as biofertilizars, Plant and Soil, 225, 521-589.
[36].    Waluyo, S.H., Lie, T.A., & Mannetje, L. (2004). Effect of phosphate on nodule rimordiaof soybean (Glycine max Merrill) in acid soils in rhizotron experiments, Indonesian Journal ofAgricultural Science, 5, 37-44, (in Farsi).
[37].    Wu, S.C., Cao, Z.H., Li Z.G., Cheung, K.C., & Wong, M.H. (2005). Effect of biofertilizer containing N-fixer, Pand K solubilizers and AM fungi on maize growth: a greenhouse trialGeoderma, 125, 155-166.
[38].    Yordanov, V., & Tsoev, T. (2000). Plant responses to drought, acclimation and stress tolerance, Photosynthica, 38(1), 171-186.