The effects of salinity and drought stress on germination, early growth and ion content in Thymbra spicata L.

Document Type : Research Paper

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Abstract

This study was conducted to investigate effects of different osmotic potential (0, -1, -2.16, -4, -5.4 and -10.9) induced by NaCl and Polyethylene Glycol-6000 (PEG) as salinity and drought stress respectively, on germination and early growth of Thymbra spicata L.The experimental design was completely randomized with three replications in which arranged as factorial. The experiment was carried out in a germinator (25°C) with 20 seeds in each Petridish. After 2 weeks germination percentage, germination rate, length of stem and root, dry and wet weight of stem and root were measured. Results showed increasing osmotic potentials decreased significantly rate and percentage of germination. Highest amounts of these indices were measured in control and lowest in osmotic potential of -10.4 bar. In similar osmotic potential, the effect of osmotic potential induced by PEG6000 was more than the effects of NaCl. Increasing of osmotic potential decreased length, wet and dry weight of root. In similar osmotic potential the effect of osmotic potential induced by NaCl was more than the effects of PEG6000 on mentioned indexes. Results also showed the high osmotic potential in both treatments affected negatively on length, wet and dry weight of stem. These indices affected more by PEG6000 than NaCl. Finally, the differences between two treatments in all measured germination indices were discussed.

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